Device

Part:BBa_K1077004

Designed by: Mike Ferguson and Joshua Abramson   Group: iGEM13_Michigan   (2013-09-16)

natural fim switch OFF orientation

Design and Construction of the new natural fim switch:

Given the importance of the LRP and IHF binding sites in the switch, and the lack of these sites in the engineered fim switch we created last year, we decided to copy the natural fim switch as close as possible. The sequence of the switch varies slightly from strain to strain in E. coli. We chose to use the natural switch sequence from E. coli CFT073 because that is the strain that has the most characterization data on hbiF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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